Low level of lytic replication in a recombinant Epstein-Barr virus carrying an origin of replication devoid of BZLF1-binding sites.
J Virol
; 78(21): 12082-4, 2004 Nov.
Article
en En
| MEDLINE
| ID: mdl-15479851
ABSTRACT
Binding of the BZLF1 viral transactivator to Epstein-Barr virus (EBV) oriLyt has been reported to be essential for viral DNA replication. We have constructed a recombinant virus (E2-oriLyt-R) in which the oriLyt BZLF1-binding sites (ZRE) were exchanged against papilloma E2-binding sites. A fusion protein between the BZLF1 protein-transactivating domain and the E2 protein-binding domain was able to reactivate lytic replication in E2-oriLyt-R. However, BZLF1 alone could also induce E2-oriLyt-R, albeit with much lower efficiency. ZRE are therefore important but not absolutely essential cis elements for lytic replication. This shows the importance of recombinants to evaluate viral functions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Replicación Viral
/
Transactivadores
/
Herpesvirus Humano 4
/
Origen de Réplica
/
Proteínas de Unión al ADN
Límite:
Humans
Idioma:
En
Revista:
J Virol
Año:
2004
Tipo del documento:
Article