Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway.
J Microbiol Methods
; 60(1): 41-6, 2005 Jan.
Article
en En
| MEDLINE
| ID: mdl-15567223
ABSTRACT
3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tolueno
/
Benzoatos
/
Pseudomonas putida
/
Colorantes Fluorescentes
Idioma:
En
Revista:
J Microbiol Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos