Transfer of a gene containing the Arg-Gly-Asp peptide prolongs the bleeding time of mice.
Biotechnol Lett
; 26(20): 1575-80, 2004 Oct.
Article
en En
| MEDLINE
| ID: mdl-15604799
ABSTRACT
Peptides containing Arg-Gly-Asp (RGD) have been used to decrease thrombosis by competitive inhibition of the integrin glycoprotein, alphaIIb/beta3a, in platelets. However, they have a short half-life in vivo. A naked plasmid, pCMV-RGD, was transferred into the skeletal muscle of mice and RGD gene expression was observed by RT-PCR. The bleeding time between control mice and RGD-transferred mice was prolonged from the 10th day to the 80th day after gene transfer while the blood glucose and serum insulin-like proteins remained at normal levels. These results provided a convenient and effective approach to relieve patients from thrombi in a single step over a relatively long period.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligopéptidos
/
Coagulación Sanguínea
/
ADN
/
Técnicas de Transferencia de Gen
/
Músculo Esquelético
Límite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Año:
2004
Tipo del documento:
Article