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Rapid monitoring of microbial contamination on herbal medicines by fluorescent staining method.
Nakajima, K; Nonaka, K; Yamamoto, K; Yamaguchi, N; Tani, K; Nasu, M.
Afiliación
  • Nakajima K; Hiya Pharmaceutical Co. Ltd, Osaka, Japan.
Lett Appl Microbiol ; 40(2): 128-32, 2005.
Article en En | MEDLINE | ID: mdl-15644112
AIMS: To apply fluorescent staining method for fast assessment of microbial quality of herbal medicines. METHODS AND RESULTS: The number of total bacteria and esterase-active bacteria on powdered traditional Chinese medicines were enumerated by fluorescent staining method using 6-carboxyfluorescein diacetate (6CFDA) and 4',6-diamidino-2-phenylindole (DAPI), and they were compared with colony-forming units (CFU). The CFU was approximately 10(3) per gram in ginseng radix, and no bacterial colonies were detected from others. However, the total bacterial number (TDC) was more than 10(7) per gram, and number of bacteria possessing esterase activity ranged from 1 to 3% of TDC. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: Many bacteria in each Chinese medicine had enzyme activity and most of them could not be detected by conventional plate counting technique. Enumeration of bacterial cells on traditional Chinese medicines by fluorescent staining method requires less than 1 h. The double staining method with 6CFDA and DAPI could be applicable to rapid microbial monitoring of crude drugs.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Contaminación de Medicamentos / Medicina de Hierbas / Colorantes Fluorescentes / Medicina Tradicional China Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals Idioma: En Revista: Lett Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Contaminación de Medicamentos / Medicina de Hierbas / Colorantes Fluorescentes / Medicina Tradicional China Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals Idioma: En Revista: Lett Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido