[Immune potency of recombinant adeno-associated virus combined with recombinant adenovirus vaccine containing HIV-1 gp120].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 18(4): 312-5, 2004 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-15650777
ABSTRACT
OBJECTIVE:
To study the immune effect of recombinant adeno-associated virus (rAAV) combined with recombinant adenovirus (rAdV) vaccine in BALB/c mice.METHODS:
The codon-modified HIV-1 gp120 gene was inserted into plasmid of adeno-associated virus and adenovirus vector separately. Then the rAAV and rAdV vaccines were constructed. BALB/c mice were immunized with rAAV and rAdV vaccines in different administration scheme. The IgG antibody was detected by ELISA and CTL response was detected by intracellular cytokine stain assay.RESULTS:
Both rAAV and rAdV vaccine could express gp120 gene; the mice primed with rAAV at week 0, 2 and boosted with rAdV at week 5, 14 and 20 elicited the strongest gp120 specific CTL and IgG antibody response.CONCLUSION:
The mice primed with rAAV and boosted with rAdV could elicit specific CTL response and IgG antibody.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína gp120 de Envoltorio del VIH
/
Adenoviridae
/
Dependovirus
/
Vacunas de ADN
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Asunto de la revista:
VIROLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
China