Modified isocratic capillary electrophoresis detection of cell-free DNA in semen.
J Assist Reprod Genet
; 21(11): 397-400, 2004 Nov.
Article
en En
| MEDLINE
| ID: mdl-15672952
ABSTRACT
PURPOSE:
The objectives were i) to analyze semen for the presence of cell-free DNA and ii) to determine the association between sperm parameters and cell-free DNA.METHODS:
Cell-free DNA in semen (N = 25 cases) were detected using the modified capillary gel electrophoresis (CE) procedure. SYBR-Gold was used to stain high (12 Kb) and low (1 Kb) molecular weight DNA fragments and the images analyzed.RESULTS:
The quantity of low-molecular weight cell-free DNA was positively correlated to rapid progression, curvilinear velocity (>40 microm/s), normal strict morphology and capacitation index. High-molecular weight cell-free DNA intensity index was negatively correlated to post-wash hyperactivation. Sperm concentration was not related to cell-free DNA quantity. The sperm freezing process did not increase cell-free DNA but reduced the more labile low-molecular weight cell-free DNA.CONCLUSIONS:
Cell-free DNA present in semen was correlated to important sperm parameters linked to normal sperm function. The data suggested the possible use of cell-free DNA as a marker of semen quality. This study reports on the novel finding of cell-free DNA released along with sperm during each ejaculation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Semen
/
Espermatozoides
/
ADN
/
Electroforesis Capilar
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
J Assist Reprod Genet
Asunto de la revista:
GENETICA
/
MEDICINA REPRODUTIVA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos