Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro.
J Microbiol Methods
; 61(1): 127-30, 2005 Apr.
Article
en En
| MEDLINE
| ID: mdl-15676202
ABSTRACT
The effect of culture medium, washing, temperature, and sonication on the infectivity of Chlamydia pneumoniae for HL cells in experiments with heparin or heparinase treatment was studied. The presence of fetal calf serum (FCS) was necessary, and simple washing of elementary bodies increased their infectivity titer. Prolonged sonication was detrimental. Detailed description of the experimental conditions is necessary in studies of chlamydial attachment and infectivity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones por Chlamydia
/
Técnicas Bacteriológicas
/
Chlamydophila pneumoniae
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Microbiol Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
Finlandia