Site-specific polymer attachment to a CCL-5 (RANTES) analogue by oxime exchange.
J Am Chem Soc
; 127(5): 1350-1, 2005 Feb 09.
Article
en En
| MEDLINE
| ID: mdl-15686348
ABSTRACT
A synthetic strategy that allows for the site-specific attachment of polymers such as poly(ethylene glycol) (PEG) to protein pharmaceuticals is described. PEG was attached to a 67-amino acid fully synthetic CCL-5 (RANTES) analogue at its GAG binding site both to reduce aggregation and to increase the circulating lifetime. Effective protection of an Aoaa chemoselective linker during peptide assembly, total chemical protein synthesis, and protein folding was achieved with an isopropylidene group. Mild deprotection of the resulting folded synthetic protein and subsequent polymer attachment occur without interference with the native folded structure and activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oximas
/
Quimiocina CCL5
/
Quimiocinas CC
Límite:
Humans
Idioma:
En
Revista:
J Am Chem Soc
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos