Improving dioxygenase stability by gene chromosome insertion: implementation in immobilized-cell systems.
Curr Microbiol
; 49(6): 390-5, 2004 Dec.
Article
en En
| MEDLINE
| ID: mdl-15696613
ABSTRACT
The immobilization of recombinant cells by using the unstable 3,4-dihydroxyphenylacetate 2,3-dioxygenase was studied as a model. Dioxygenase activity and cell viability were compared in immobilized-cell systems and cells in suspension. Immobilization increased enzyme stability and the efficient degradation of 3,4-dihydroxyphenylacetate. The stability of the cloned enzyme and the viability of the immobilized recombinant cells were well maintained for at least 15 days. We used the strain Escherichia coli CC118-D in which the hpaB gene from Klebsiella pneumoniae, coding for the subunit of 3,4-dihydroxyphenylacetate 2,3-dioxygenase, was inserted into the chromosome. This study has demonstrated that the implementation of E. coli CC118-D in a pilot-scale bioreactor resulted in a 100% stabilization of dioxygenase activity, and could be a useful tool for bioremediation processes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Células Inmovilizadas
/
Dioxigenasas
/
Escherichia coli
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Curr Microbiol
Año:
2004
Tipo del documento:
Article
País de afiliación:
España