Antibiotic susceptibility of bacteria isolated from pasteurized milk and characterization of macrolide-lincosamide-streptogramin resistance genes.
J Food Prot
; 68(2): 347-52, 2005 Feb.
Article
en En
| MEDLINE
| ID: mdl-15726980
ABSTRACT
The presence of antibiotic-resistant bacteria in pasteurized milk was detected by plating 18 milk samples on selective media containing beta-lactams, macrolides, or a glycopeptide. Most samples contained gram-positive bacteria that grew on agar plates containing oxacillin, erythromycin, and/or spiramycin. The disk-diffusion method confirmed resistance to erythromycin and/or spiramycin in 86 and 65% of the coryneform bacteria and Micrococcaceae tested, respectively. PCR and sequence analysis revealed the presence of an ermC gene in 2 of the 25 Micrococcaceae strains investigated for their resistance to erythromycin and/or spiramycin. None of the 14 corynebacteria strains resistant to erythromycin and/or spiramycin harbored the erm(X) gene. No gene transfer could be demonstrated between the two erm(C) staphylococcal isolates and recipient strains of Enterococcus faecalis JH2-2 or Staphylococcus aureus 80CR5.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Seguridad de Productos para el Consumidor
/
Corynebacterium
/
Leche
/
Manipulación de Alimentos
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Micrococcaceae
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Antibacterianos
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Food Prot
Año:
2005
Tipo del documento:
Article
País de afiliación:
Francia