Human receptor Smoothened, a mediator of Hedgehog signalling, expressed in its native conformation in yeast.
FEBS Lett
; 579(6): 1529-33, 2005 Feb 28.
Article
en En
| MEDLINE
| ID: mdl-15733868
ABSTRACT
Though the role of Hedgehog (Hh) signalling in patterning and differentiation during development is well established, the underlying signal transduction mechanisms remain obscure. This is the first report on the overexpression of the human Hh signalling receptor Smoothened (hSmo) in Saccharomyces cerevisiae and Pichia pastoris. We show that hSmo is expressed in both types of yeast in its native conformational state. The first purification presented here will allow the characterisation of hSmo expressed in yeast, and the scale-up of hSmo production enabling structural studies to develop new therapeutic approaches against tumors and neurodegenerative diseases induced by Hh signalling dysfunction.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pichia
/
Saccharomyces cerevisiae
/
Transducción de Señal
/
Transactivadores
/
Receptores Acoplados a Proteínas G
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2005
Tipo del documento:
Article
País de afiliación:
Francia