Molecular profiling of Bacteroides spp. in human feces by PCR-temperature gradient gel electrophoresis.
J Microbiol Methods
; 61(3): 413-7, 2005 Jun.
Article
en En
| MEDLINE
| ID: mdl-15767017
ABSTRACT
A group-specific PCR-based temperature gradient gel electrophoresis (TGGE) method was developed to study the population composition of genus Bacteroides in human gut. Highly reproducible and well-separated bands in TGGE fingerprints of ten unrelated human fecal samples showed complex and host-specific Bacteroides species composition. Dynamic monitoring over 22 months of samples from one healthy 10-year-old boy indicated a relatively stable population profile of Bacteroides. The species identity of each band in TGGE gel of this boy was also resolved via comigration analysis of sequenced inserts in a Bacteroides group-specific clone library. This work provides a rapid and effective technique for analyzing the species composition of Bacteroides in human gut.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacteroides
/
Reacción en Cadena de la Polimerasa
/
Heces
Límite:
Child
/
Humans
/
Male
Idioma:
En
Revista:
J Microbiol Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
China