A comprehensive radiation hybrid map of the bovine genome comprising 5593 loci.
Genomics
; 85(4): 413-24, 2005 Apr.
Article
en En
| MEDLINE
| ID: mdl-15780744
ABSTRACT
A bovine whole genome 7000-rad radiation hybrid (RH) panel, SUNbRH(7000-rad), was constructed to build a high-resolution RH map. The Shirakawa-USDA linkage map served as a scaffold to construct a framework map of 3216 microsatellites on which 2377 ESTs were ordered. The resulting RH map provided essentially complete coverage across the genome, with 1 cR7000 corresponding to 114 kb, and a cattle-human comparative map of 1716 bovine genes and sequences annotated in the human genome, which covered 79 and 72% of the bovine and human genomes, respectively. We then integrated the bovine RH and comparative maps with BAC fingerprint information in to construct a detailed, BAC-based physical map covering a reported 40-cM quantitative trait locus region for intramuscular fat or "marbling" on BTA 4. In summary, the new, high-resolution SUNbRH7000-rad, comparative, Shirakawa-USDA linkage, and BAC fingerprint maps provide a set of genomic tools for fine mapping regions of interest in cattle.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bovinos
/
Genoma
/
Repeticiones de Microsatélite
/
Mapeo de Híbrido por Radiación
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón