[Inter-laboratory evaluation studies for establishment of notified ELISA methods for allergic substances (buckwheat)].
Shokuhin Eiseigaku Zasshi
; 45(6): 313-8, 2004 Dec.
Article
en Ja
| MEDLINE
| ID: mdl-15794089
ABSTRACT
Inter-laboratory evaluation studies were conducted for the ELISA methods for allergic substances (buckwheat). Extracts of snack, bun and udon spiked with buckwheat standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate at 10 laboratories. Coefficients of variation (CVs) of the ELISA methods using a Buckwheat Protein ELISA Kit (Buckwheat kit) and a FASTKIT Buckwheat ELISA kit (Buckwheat ELISA kit) were mostly below 10%. Mean recoveries of the buckwheat standard protein from the food extracts were over 40% in the two ELISA methods. Repeatability relative standard deviations of buckwheat standard protein in three food extracts were in the ranges of 6.8-78.5% and 5.0-33.9% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. Reproducibility relative standard deviations of buckwheat standard protein in three food extracts were 11.9-69.5% and 16.5-34.1% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. The detection limits of both ELISA methods were 1 ng/mL in sample solutions. These results suggest that the notified ELISA methods are reliable and reproducible for the inspection of buckwheat protein levels in extracts of snack, bun and udon.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Alérgenos
/
Fagopyrum
/
Análisis de los Alimentos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
País/Región como asunto:
Asia
Idioma:
Ja
Revista:
Shokuhin Eiseigaku Zasshi
Año:
2004
Tipo del documento:
Article
País de afiliación:
Japón