Development of an oral biosensor for salivary amylase using a monodispersed silver for signal amplification.
Anal Biochem
; 340(1): 136-44, 2005 May 01.
Article
en En
| MEDLINE
| ID: mdl-15802139
ABSTRACT
An amperometric biosensor for monitoring the level of protein amylase in human saliva is described. A novel design and the preparation of amylase antibodies and antigens, essential for the development of the biosensor, are reported. The biosensor sensing elements comprise a layer of salivary antibody (or antigen) self-assembled onto Au-electrode via covalent attachment. Molecular recognition between the immobilized antibody and the salivary amylase proteins was monitored via an electroactive indicator (e.g., K(3)Fe(CN)(6)) or a monodispersed silver layer present in solution or electrochemically deposited onto the solid electrode. This electroactive indicator was oxidized or reduced and the resulting current change provided the analytical information about the concentration of the salivary proteins. The limit of detection of 1.57 pg/ml was obtained, in comparison to detection limits of 4.95 pg/ml obtained using potassium ferrocyanide as the redox probe and 10 ng/ml obtained using enzyme-linked immunosorbent assay. Cross-reactivity was tested against cystatin antibodies and was found to be less than 2.26%.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saliva
/
Plata
/
Técnicas Biosensibles
/
Amilasas
/
Boca
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos