Defective infectious particles and rare packaged genomes produced by cells carrying terminal-repeat-negative epstein-barr virus.
J Virol
; 79(12): 7641-7, 2005 Jun.
Article
en En
| MEDLINE
| ID: mdl-15919916
ABSTRACT
The Epstein-Barr virus (EBV) lytic program includes lytic viral DNA replication and the production of a viral particle into which the replicated viral DNA is packaged. The terminal repeats (TRs) located at the end of the linear viral DNA have been identified as the packaging signals. A TR-negative (TR(-)) mutant therefore provides an appropriate tool to analyze the relationships between EBV DNA packaging and virus production. Here, we show that supernatants from lytically induced 293 cells carrying TR mutant EBV genomes (293/TR(-)) contain large amounts of viral particles devoid of viral DNA which are nevertheless able to bind to EBV target cells. This shows that viral DNA packaging is not a prerequisite for virion formation and egress. Rather surprisingly, supernatants from lytically induced 293/TR(-) cells also contained rare infectious viruses carrying the viral mutant DNA. This observation indicates that the TRs are important but not absolutely essential for virus encapsidation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virión
/
Genoma Viral
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Herpesvirus Humano 4
/
Ensamble de Virus
/
Secuencias Repetidas Terminales
/
Virus Defectuosos
Límite:
Humans
Idioma:
En
Revista:
J Virol
Año:
2005
Tipo del documento:
Article
País de afiliación:
Alemania