An acetyl-CoA synthetase not encoded by the facA gene is expressed under carbon starvation in Phycomyces blakesleeanus.
Res Microbiol
; 156(5-6): 663-9, 2005.
Article
en En
| MEDLINE
| ID: mdl-15921892
ABSTRACT
Two forms of acetyl-CoA synthetase (ACS1 and ACS2) have been detected in Phycomyces blakesleeanus. ACS1, encoded by the gene facA, was induced by acetate and repressed by glucose at the transcriptional level. ACS2, not encoded by the gene facA, was detected as a response to carbon starvation both in the wild type and in an facA(-) mutant. Both enzymes were purified and characterized. They can use acetate and propionate as substrates. ACS2 is a much more stable enzyme than ACS1. After 60 min incubation at 55 degrees C, ACS2 retained 50% of its activity whereas ACS1 only retained 3%. The optimum temperature was 50 degrees C for ACS2 and 30 degrees C for ACS1.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Phycomyces
/
Regulación Fúngica de la Expresión Génica
/
Coenzima A Ligasas
Idioma:
En
Revista:
Res Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
España