The expression of prion protein (PrP(C)) in the megakaryocyte lineage.
J Thromb Haemost
; 3(6): 1266-73, 2005 Jun.
Article
en En
| MEDLINE
| ID: mdl-15946217
ABSTRACT
BACKGROUND:
Cellular prion protein (PrP(C)) is a naturally occurring protein in normal individuals which adopts an abnormal conformation, termed scrapie prion protein (PrP(Sc)) that is associated with disease. There is great concern that clinically asymptomatic variant Creutzfeldt-Jacob disease (vCJD) may transmit PrP(Sc) in blood transfusion products. PrP(C) is widely expressed and has been found in human blood. The majority of cellular borne PrP(C) is associated with platelets (84%). Although PrP(C) mRNA has been demonstrated in platelets, the quantity is unknown and may not reflect the total PrP(C) present.OBJECTIVE:
To investigate the expression of PrP(C) in the megakaryocyte lineage.METHODS:
The expression of PrP(C) was studied in CD34+ cells, cultured megakaryocytes and platelets using electron microscopy, flow cytometry, semi-quantitative RT-PCR and immunofluorescence confocal microscopy. RESULTS ANDCONCLUSIONS:
The expression of PrP(C) appeared to increase with differentiation and polyploidization in the megakaryocyte lineage. PrP(C) was located within platelet alpha-granules and its source is likely to be from megakaryocyte precursors. If PrP(Sc) has a similar distribution, these results have implications for the selection of blood donors and preparation of cell-depleted blood products.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Megacariocitos
/
Proteínas PrPC
Límite:
Humans
Idioma:
En
Revista:
J Thromb Haemost
Asunto de la revista:
HEMATOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Reino Unido