Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues.
Virchows Arch
; 447(3): 573-85, 2005 Sep.
Article
en En
| MEDLINE
| ID: mdl-15968546
ABSTRACT
The present study is based on the initiative for quality assurance in pathology of the German Society of Pathology and the Professional Association of German Pathologists. Four panel laboratories with experience and expertise in polymerase chain reaction (PCR) detection of Mycobacterium tuberculosis were selected to establish the prerequisites for continuous external laboratory trials, in particular, by providing pre-tested specimens and evaluation criteria for participating institutes. In the first step, the four panel laboratories performed an internal trial to test their own reliability and reproducibility. Paraffin sections and DNA preparations from 34 tissues (25 clinical specimens and 9 controls) totalling to 66 samples were evaluated by each panel institute according to their own protocols. The methodologies differed and are described in detail. Despite these differences, a high degree of inter-laboratory reliability was achieved. In this report, we summarise our results including the correlation with the histology and provide recommendations for applying PCR-based methodology for the detection of mycobacterial DNA in surgical specimens. Supplementary data are available online at http//www.charite.de/ch/patho (rubric "Forschung"). Pre-tested specimens are now available for the external trial and can be ordered from the steering institute via Oligene (http//www.oligene.com/). All molecular pathology laboratories are invited to participate in this quality assurance initiative.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Patología Clínica
/
Garantía de la Calidad de Atención de Salud
/
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Mycobacterium tuberculosis
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
/
Guideline
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Virchows Arch
Asunto de la revista:
BIOLOGIA MOLECULAR
/
PATOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Alemania