Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans.
Biotechnol Lett
; 27(8): 545-50, 2005 Apr.
Article
en En
| MEDLINE
| ID: mdl-15973487
ABSTRACT
The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg-1.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacillus
/
ADN Bacteriano
/
Endo-1,4-beta Xilanasas
Idioma:
En
Revista:
Biotechnol Lett
Año:
2005
Tipo del documento:
Article
País de afiliación:
Argentina