Accelerating bacterial identification by infrared spectroscopy by employing microarray deposition of microorganisms.
Foodborne Pathog Dis
; 1(3): 172-7, 2004.
Article
en En
| MEDLINE
| ID: mdl-15992277
ABSTRACT
A microarray method for the deposition of bacteria onto an agar slide was developed to accelerate the formation of microcolonies. Representative microarrays each consisting of 40 micro-spots of five replicates of eight foodborne bacteria (Yersinia enterocolitica, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli) were printed on a Brain Heart Infusion (BHI) agar slide using a contact micro-spotting robotic system. Within 3 h, sufficient bacterial cells were obtained to allow accurate identification of the microorganism by infrared spectroscopy. This approach allows a "complete-in-a-single-day" analysis of a large array of samples.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Espectroscopía Infrarroja por Transformada de Fourier
/
Análisis por Micromatrices
/
Microbiología de Alimentos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Foodborne Pathog Dis
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
MICROBIOLOGIA
/
PARASITOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos