Genomic analysis of heat-shock factor targets in Drosophila.
Genome Biol
; 6(7): R63, 2005.
Article
en En
| MEDLINE
| ID: mdl-15998452
ABSTRACT
We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genómica
/
Proteínas de Drosophila
/
Drosophila melanogaster
/
Proteínas de Choque Térmico
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Reino Unido