Identification of the pathological prion protein allotypes in scrapie-infected heterozygous bank voles (Clethrionomys glareolus) by high-performance liquid chromatography-mass spectrometry.
J Chromatogr A
; 1081(1): 122-6, 2005 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-16013608
ABSTRACT
Cerebral formation of the pathological isoform of the prion protein (PrP) is a crucial molecular event in prion diseases. The bank vole (Clethrionomys glareolus) is a rodent species highly susceptible to natural scrapie. The PrP gene of bank vole is polymorphic (Met/Ile) at codon 109. Here we show that homozygous 109Met/Met voles have incubation times shorter than heterozygous 109Met/Ile voles after experimental challenge with three different scrapie isolates. An HPLC-MS/MS method was optimized and applied to investigate whether in heterozygous animals both PrP allotypes are able to undergo pathological conversion. The results demonstrate that both allotypes of the prion protein participate to pathological deposition.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Scrapie
/
Priones
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr A
Año:
2005
Tipo del documento:
Article
País de afiliación:
Italia