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Suppression of polyethylene particle-induced osteolysis by exogenous osteoprotegerin.
von Knoch, Fabian; Heckelei, Anja; Wedemeyer, Christian; Saxler, Guido; Hilken, Gero; Brankamp, Jochen; Sterner, Thomas; Landgraeber, Stefan; Henschke, Frank; Löer, Franz; von Knoch, Marius.
Afiliación
  • von Knoch F; Department of Orthopaedic Surgery, Kantonsspital Chur, Loestrasse 170, 7000 Chur, Switzerland. fabianvonknoch@yahoo.com
J Biomed Mater Res A ; 75(2): 288-94, 2005 Nov 01.
Article en En | MEDLINE | ID: mdl-16088891
ABSTRACT
Alterations of the key regulators of osteoclastogenesis, receptor activator of NF-kappaB (RANK), RANK ligand (RANKL), and osteoprotegerin (OPG) have been implicated in wear particle-induced osteolysis, the most common cause for implant failure in total joint replacements. This study investigated the effect of exogenous OPG on ultra-high-molecular-weight polyethylene (UHMWPE) particle-induced osteolysis. The murine calvarial osteolysis model was utilized in 28 C57BL/6J mice randomized to four groups. Group I underwent sham surgery only, group II received UHMWPE particles, and group III and IV particles and subcutaneous OPG starting from day 0 (group III) or day 5 (group IV) until sacrifice. After 2 weeks, calvaria were prepared for histology and histomorphometry. Bone resorption was measured within the midline suture using Giemsa staining and osteoclast numbers were determined using TRAP staining. UHMWPE particle implantation resulted in grossly pronounced osteoclastogenesis and bone resorption. Both immediate and delayed treatment with OPG counteracted these particle-induced effects significantly, suppressing osteoclast formation and bone resorption (p < 0.001 and p < 0.001, respectively). In conclusion, exogenous OPG markedly suppressed UHMWPE particle-induced osteolysis in a murine calvarial model. This important finding underscores the crucial significance of the OPG-RANKL-RANK signaling in wear particle-induced osteolysis. Exogenous OPG may prove an effective treatment modality for wear debris-mediated periprosthetic osteolysis after total joint arthroplasty.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteólisis / Polietilenos / Falla de Prótesis / Glicoproteínas / Receptores del Factor de Necrosis Tumoral / Receptores Citoplasmáticos y Nucleares Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2005 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteólisis / Polietilenos / Falla de Prótesis / Glicoproteínas / Receptores del Factor de Necrosis Tumoral / Receptores Citoplasmáticos y Nucleares Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2005 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA