Weak D type 1.1 exemplifies another complexity in weak D genotyping.
Transfusion
; 45(10): 1568-73, 2005 Oct.
Article
en En
| MEDLINE
| ID: mdl-16181206
ABSTRACT
BACKGROUND:
Weak D expression is caused by a large number of RHD alleles. Increasingly recommendations for D+ or D- transfusions are based on polymerase chain reaction (PCR) identification of certain RHD alleles. Possible sources of error are rare D variants that are inadvertently carrying known polymorphisms of frequent weak D types. STUDY DESIGN ANDMETHODS:
Weak D donors were checked by direct column agglutination. In donors with unusually weak expression of D, the molecular weak D type was determined by weak D PCR and nucleotide sequencing. The serologic profile of a weak D type 1 variant was determined by agglutination serology and flow cytometry.RESULTS:
Several donors in whom direct agglutination barely revealed any D expression were shown to carry the new RHD(L18V,V270G) allele dubbed weak D type 1.1. Initially, such donors had been mistyped as weak D type 1 by PCR. In a systematic study, weak D type 1.1 was shown to be present in 7 of 23 donors with very weak D expression who all lived in a restricted area of Northern Germany. Although weak D type 1.1 was typed D- or barely D+ by direct agglutination, it was easily detected by antiglobulin technique and was shown to carry about 600 antigens D per red blood cell.CONCLUSION:
The observation of weak D type 1.1 with its distinct phenotype pinpointed to two general problems of current RHD genotyping strategies Mistyping of alleles with additional mutations and striking geographic variation of the allele distributions.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo Rh-Hr
/
Tipificación y Pruebas Cruzadas Sanguíneas
/
Etnicidad
Tipo de estudio:
Prognostic_studies
Límite:
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
Transfusion
Año:
2005
Tipo del documento:
Article
País de afiliación:
Alemania