Characterization of an aminopeptidase in cerebrospinal fluid. Structure elucidation of enzyme hydrolysis products of synthetic methionine-enkephalin by reversed-phase high-performance liquid chromatography and mass spectrometry.
J Chromatogr
; 574(2): 189-96, 1992 Feb 14.
Article
en En
| MEDLINE
| ID: mdl-1618949
ABSTRACT
An aminopeptidase was found in canine cerebrospinal fluid via the presence of two products Y, which has an [M + H]+ ion at m/z 182; and GGFM, which has an [M + H]+ ion at m/z 411. The linked scan at a constant ratio of the magnetic field to the electric field of the GGFM [M + H]+ ion at m/z 411 generates product ions at m/z 120, 150, 266, 297, 354, 357, and 411. That aminopeptidase was bestatin-sensitive (BSAP = bestatin-sensitive aminopeptidase), and had a half-time for disappearance of 60 min, maximum velocity of 1.08 nmol ml-1 min-1, and Michaelis constant of 0.26 nM.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Encefalina Metionina
/
Aminopeptidasas
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr
Año:
1992
Tipo del documento:
Article