Preparative isolation and purification of polymethoxylated flavones from Tangerine peel using high-speed counter-current chromatography.
J Chromatogr A
; 1090(1-2): 188-92, 2005 Oct 07.
Article
en En
| MEDLINE
| ID: mdl-16196149
ABSTRACT
Several polymethoxylated flavones including nobiletin, 3,5,6,7,8,3',4'-heptamethoxyflavone, tangeretin and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone were separated from Tangerine peel (Juhong in Chinese) by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10.811, v/v). Then, 26 mg of nobiletin, 6 mg of 3,5,6,7,8,3',4'-heptamethoxyflavone, 35 mg of tangeretin and 11 mg of 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone could be obtained from 150 mg crude extracts and their purities were 98.6%, 95.9%, 99.8% and 96.8%, respectively. All these constituents were identified by EI-MS and 1H NMR.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citrus
/
Distribución en Contracorriente
/
Flavonas
/
Frutas
Idioma:
En
Revista:
J Chromatogr A
Año:
2005
Tipo del documento:
Article
País de afiliación:
China