Synthesis of functional protein in liposome.
J Biosci Bioeng
; 92(6): 590-3, 2001.
Article
en En
| MEDLINE
| ID: mdl-16233152
ABSTRACT
The liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.510.08 was used to entrap cell-free protein synthesis reaction mixture. The synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from the liposome on flow cytometry analysis and fluorescence microscopy. The protein synthesized in the liposome is hence functional.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
J Biosci Bioeng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón