Production of human metabolites of cyclosporin A, AM1, AM 4 N and AM 9, by microbial conversion.
J Biosci Bioeng
; 99(4): 390-5, 2005 Apr.
Article
en En
| MEDLINE
| ID: mdl-16233807
ABSTRACT
In humans, cyclosporin A (CyA) is primarily metabolized to two hydroxylated (AM1 and AM 9) and one N-demethylated (AM 4 N) derivative. To produce these derivatives, 1237 actinomycetes were screened for their ability to convert CyA. Among them, 89 strains (7.2%) produced these derivatives from CyA. Finally, Dactylosporangium variesporum IFO 14104, Actinoplanes sp. ATCC 53771 and Streptosporangium sp. AF 935 were selected for the production of AM1, AM 4 N, and AM 9, respectively. Composition of the production medium and the incubation period with CyA were important for obtaining a high yield of CyA derivatives. Large-scale microbial conversion of CyA (1.5 g) using a 30-l jar fermentor yielded 288 mg of AM1 (19.2%), 147 mg of AM 4 N (9.8%) and 115 mg of AM 9 (7.7%).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
/
Ciclosporina
/
Actinobacteria
Límite:
Humans
Idioma:
En
Revista:
J Biosci Bioeng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón