Molecular genetic analysis for Ax phenotype of the ABO blood group system in Chinese.
Vox Sang
; 89(4): 251-6, 2005 Nov.
Article
en En
| MEDLINE
| ID: mdl-16262759
ABSTRACT
BACKGROUND AND OBJECTIVES:
To elucidate the molecular genetic background of the Ax phenotype in the Chinese population. MATERIALS ANDMETHODS:
The ABO genes of eight Ax phenotype samples, four Ax and four AxB, were amplified by polymerase chain reaction (PCR) and were cloned, along with those of 10 random A(1) Chinese subjects. We analysed the ABO gene transcript structure and the sequences of two exons and one intron at the ABO locus.RESULTS:
Among the four Ax phenotype samples, we identified one Ax02, two Ax03 and one novel Ax allele with the 543G > T mutation in the A102 background. Two of five family members also carry the allele. Of the four AxB phenotypes, one was designated as cis-AB-1/B101; the other three were shown to carry one B allele and one O with the nt261G deletion. The B alleles of the latter three were identical to B101 except for single point mutation at nt700C > G, nt640A > G and nt641T > C, respectively. The novel B101-like alleles were first associated with A(weak)B phenotypes.CONCLUSIONS:
Two ABO*B(A) alleles and an Ax allele clearly differ from all previously reported ABO alleles, suggesting that the molecular genetic background of Ax is heterogeneous in the Chinese population.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo ABO
/
Exones
/
Mutación Puntual
/
Sitios de Carácter Cuantitativo
/
Alelos
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
/
Male
País/Región como asunto:
Asia
Idioma:
En
Revista:
Vox Sang
Año:
2005
Tipo del documento:
Article
País de afiliación:
China