[The expression of recombinant protein of human interleukin-24 and its anti-tumor mechanism].
Sheng Wu Gong Cheng Xue Bao
; 21(5): 719-24, 2005 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-16285511
ABSTRACT
The hIL24 cDNA sequence was cloned into prokaryotic high expressive vector pET-21a(+) and recombinant hIL24 was expressed in E. coli with IPTG induction. The purified recombinant hIL24 exhibits following functions in HeLa cell inhibiting cell growth, inducing apoptosis, inducing PMBC to secrete IL-6, TNF-alpha, IFN-r and inhibiting blood vessel formation. Our preliminary results suggest that the apoptosis induced by rhIL24 is through down-regulating expression of anti-apoptosis factor Bcl-2 and activation of mitochondria apoptosis pathway.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes
/
Interleucinas
/
Apoptosis
Límite:
Female
/
Humans
Idioma:
Zh
Revista:
Sheng Wu Gong Cheng Xue Bao
Asunto de la revista:
BIOTECNOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
China