The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development.
Nature
; 438(7068): 671-4, 2005 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-16319892
ABSTRACT
MicroRNAs (miRNAs) are an abundant class of gene regulatory molecules (reviewed in refs 1, 2). Although computational work indicates that miRNAs repress more than a third of human genes, their roles in vertebrate development are only now beginning to be determined. Here we show that miR-196 acts upstream of Hoxb8 and Sonic hedgehog (Shh) in vivo in the context of limb development, thereby identifying a previously observed but uncharacterized inhibitory activity that operates specifically in the hindlimb. Our data indicate that miR-196 functions in a fail-safe mechanism to assure the fidelity of expression domains that are primarily regulated at the transcriptional level, supporting the idea that many vertebrate miRNAs may function as a secondary level of gene regulation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transactivadores
/
Proteínas de Homeodominio
/
Regulación del Desarrollo de la Expresión Génica
/
MicroARNs
/
Extremidades
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Nature
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos