Endothelial cell function on 2D and 3D micro-fabricated polymer scaffolds: applications in cardiovascular tissue engineering.
J Biomater Sci Polym Ed
; 17(1-2): 37-51, 2006.
Article
en En
| MEDLINE
| ID: mdl-16411597
ABSTRACT
Polymeric structures of a polylactide-polycaprolactone blend were micro-fabricated using the Pressure Assisted Microsyringe (PAM) system. Human umbilical vein endothelial cells were cultured on the scaffolds, and apoptosis, cell adhesion, proliferation and metabolism were evaluated. In addition, more specific indicators of endothelial cell function, namely nitric oxide and endothelin production, were also assessed. Thin films of the blend, as well as gelatine-coated glass slides (as controls) were used. The results show that as far as adhesion, apoptosis and metabolism are concerned, the scaffolds do not interfere with cell function compared with gelatin controls. However, the nitric oxide/endothelin ratio was higher than that observed on the gelatin films, suggesting that the scaffolds could be used for engineering small diameter blood vessels without risk of occlusion.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Sistema Cardiovascular
/
Ingeniería de Tejidos
/
Células Endoteliales
Límite:
Humans
Idioma:
En
Revista:
J Biomater Sci Polym Ed
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM