Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors.
Biophys J
; 90(8): 2970-7, 2006 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-16443650
ABSTRACT
Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern 1), reliable functionalization of micromechanical cantilever arrays with proteoliposomes using ink jet spotting; 2), successful detection of the prosthetic retinal removal (bleaching) from the bacteriorhodopsin protein by measuring the induced nanomechanical surface stress change; and 3), the quantitative response thereof, which depends linearly on the amount of removed retinal. Our results show this technique to be a potential tool to measure membrane protein-based receptor-ligand interactions and conformational changes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacteriorodopsinas
/
Técnicas Biosensibles
Idioma:
En
Revista:
Biophys J
Año:
2006
Tipo del documento:
Article
País de afiliación:
Suiza