Detection of equine herpesvirus-specific effector and memory cytotoxic immunity in the equine upper respiratory tract.
Vet Immunol Immunopathol
; 111(1-2): 117-25, 2006 May 15.
Article
en En
| MEDLINE
| ID: mdl-16472871
ABSTRACT
Immunological protection of horses from equine herpesvirus-1 (EHV-1) infection and disease depends on the cooperation of virus-specific humoral and cellular immune responses. EHV-specific mucosal immunity may be an important component of such immune responses. This study demonstrates the induction of anti-EHV cytotoxic cellular immune responses in various mucosal and systemic lymphoid tissues associated with the upper respiratory tract (URT) of the horse. Four young horses (1-2 years of age) were inoculated intranasally with the Army 183 strain of EHV-1 and euthanized 1 week later. One untreated foal served as a non-infected control. Mucosa-associated tonsillar tissues, draining lymph nodes and PBMC were harvested. Virus-specific memory and effector cytolytic activity were individually assessed using 4 h chromium release assays, with and without in vitro restimulation with EHV-1, respectively. EHV-specific cytotoxic activity was detected ex vivo in several URT-associated mucosal lymphoid tissues of horses, particularly within the lining of the nasopharynx, a principal site of EHV-1 replication. This activity was also detected in the circulation of some horses 1 week post-challenge. Virus-specific memory cytotoxic activity was elevated in the circulation, and detectable in the draining lymph nodes of all horses following challenge infection.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades Respiratorias
/
Herpesvirus Équido 1
/
Infecciones por Herpesviridae
/
Enfermedades de los Caballos
/
Tejido Linfoide
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos