Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides.
Folia Microbiol (Praha)
; 50(5): 415-20, 2005.
Article
en En
| MEDLINE
| ID: mdl-16475501
ABSTRACT
The Agrobacterium tumefaciens-mediated transformation of the zygomycetous fungus Mucor circinelloides is described. A method was also developed for the hygromycin B-based selection of Mucor transformants. Transformation with the hygromycin B phosphotransferase gene of Escherichia coli controlled by the heterologous Aspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transformación Genética
/
Agrobacterium tumefaciens
/
Mucor
Idioma:
En
Revista:
Folia Microbiol (Praha)
Año:
2005
Tipo del documento:
Article
País de afiliación:
Hungria