Interfacing capillary gel microfluidic chips with infrared laser desorption mass spectrometry.
J Am Soc Mass Spectrom
; 17(3): 469-74, 2006 Mar.
Article
en En
| MEDLINE
| ID: mdl-16480892
ABSTRACT
We report on the fabrication and performance of a gel microfluidic chip interfaced to laser desorption/ionization (LDI) mass spectrometry with a time-of-flight mass analyzer. The chip was fabricated from poly(methylmethacrylate) with a poly(dimethyl siloxane) cover. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed in the channel of the microfluidic chip. After electrophoresis, the cover was removed and either the PDMS chip or the PMMA cover was mounted in a modified MALDI ion source for analysis. Ions were formed by irradiating the channel with 2.95 microm radiation from a pulsed optical parametric oscillator (OPO), which is coincident with IR absorption by N-H and O-H stretch of the gel components. No matrix was added. The microfluidic chip design allowed a decrease in the volume of material required for analysis over conventional gel slabs, thus enabling improvement in the detection limit to a pmol level, a three orders of magnitude improvement over previous studies in which desorption was achieved from an excised section of a conventional gel.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrofotometría Infrarroja
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Ensayo Cometa
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Electroforesis por Microchip
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Técnicas Analíticas Microfluídicas
/
Rayos Láser
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos