Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis.
J Chromatogr B Analyt Technol Biomed Life Sci
; 833(2): 158-64, 2006 Apr 03.
Article
en En
| MEDLINE
| ID: mdl-16490406
ABSTRACT
Two non-self-complementary 17-mer double-stranded DNA (dsDNA) with four different central base pairs were designed to systematically investigate the binding affinity and sequence specificity of berberine with dsDNA by capillary zone electrophoresis (CZE). The data analysis with the Kenndler model proved only low affinity between dsDNA and berberine and suggested some weak binding preference of berberine for AATT-containing to GGCC-containing dsDNA. The binding constant, Ka, between berberine and dsDNA(AB) was about (1.0 +/- 0.7) x 10(3) M(-1). In addition, the separation of single-stranded DNA (ssDNA) from dsDNA under simple electrophoretic conditions enabled CZE to be a potentially alternative tool to check the extent of DNA annealing, which is usually done by the time-consuming and labor-intensive slab electrophoresis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Berberina
/
ADN
/
Electroforesis Capilar
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
China