[Preliminary identification and characterization of in vitro cultured bone marrow stromal cells for their bioactivity and osteogenic potential].
Nan Fang Yi Ke Da Xue Xue Bao
; 26(1): 111-3, 2006 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-16495191
ABSTRACT
OBJECTIVE:
To induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs.METHODS:
Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity.RESULT:
BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results.CONCLUSION:
BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Osteogénesis
/
Células de la Médula Ósea
/
Diferenciación Celular
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Año:
2006
Tipo del documento:
Article
País de afiliación:
China