[Preliminary identification and characterization of in vitro cultured bone marrow stromal cells for their bioactivity and osteogenic potential].

ABSTRACT

OBJECTIVE: To induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs. METHODS: Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity. RESULT: BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results. CONCLUSION: BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.