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Effects of activated polymorphonuclear leukocytes on vascular smooth muscle tone.
Mehta, J L; Lawson, D L; Nicolini, F A; Ross, M H; Player, D W.
Afiliación
  • Mehta JL; Department of Medicine, University of Florida College of Medicine, Gainesville.
Am J Physiol ; 261(2 Pt 2): H327-34, 1991 Aug.
Article en En | MEDLINE | ID: mdl-1652210
Unstimulated polymorphonuclear leukocytes (PMNLs) release nitric oxide or a like material that relaxes vascular tissues. To determine the effects of activated PMNLs on vascular tone, precontracted rat aortic rings were exposed to ionophore A23187-treated PMNLs. Whereas "unstimulated" PMNLs caused 29 +/- 4% relaxation, "stimulated" PMNLs caused initial contraction followed by 90 +/- 7% relaxation of aortic rings. Indomethacin or the 5-lipoxygenase blocker piriprost had no effect on PMNL-induced initial contraction or subsequent relaxation. However, initial contraction was abolished and the subsequent vasorelaxation attenuated (22 +/- 5%) by the superoxide radical scavenger superoxide dismutase (SOD), suggesting that release of superoxide radicals may have induced vascular contraction and caused endothelial damage that would permit unopposed vasorelaxant effect of PMNLs. To examine this hypothesis, aortic rings were exposed to superoxide radicals (generated by xanthine plus xanthine oxidase, X + XO) or manually deendothelialized. These rings revealed marked relaxation (78 +/- 6 and 85 +/- 6%, respectively) in response to unstimulated PMNLs. These observations suggest that stimulated PMNLs exert an initial vasoconstrictor effect and a subsequent vasorelaxant effect in response to release of superoxide radicals and nitric oxide, respectively. Arachidonate metabolites or 5-lipoxygenase products do not appear to be important in the actions of PMNLs on vascular smooth muscle.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasoconstricción / Músculo Liso Vascular / Neutrófilos Límite: Animals Idioma: En Revista: Am J Physiol Año: 1991 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasoconstricción / Músculo Liso Vascular / Neutrófilos Límite: Animals Idioma: En Revista: Am J Physiol Año: 1991 Tipo del documento: Article Pais de publicación: Estados Unidos