Physostigmine modulation of acetylcholine currents in COS cells transfected with mouse muscle nicotinic receptor.
Neurosci Lett
; 401(1-2): 20-4, 2006 Jun 19.
Article
en En
| MEDLINE
| ID: mdl-16530961
ABSTRACT
Physostigmine (Phy), a reversible inhibitor of acetylcholine (ACh) esterase (AChE), may also act as a low potency agonist and a modulator of the nicotinic receptor. The actions of Phy on mouse muscle nicotinic receptors in the COS-7 cell line were studied by the patch-clamp technique. Currents were recorded in the whole-cell mode 3-7 days after cell transfection by plasmids coding alphabetagammadelta combination of receptor subunits. The application of ACh to cells clamped at -10 mV produced inward currents which displayed desensitization. The application of Phy in concentrations up to 1 x 10(-3) M did not give reliable specific whole-cell membrane responses. The application of Phy in concentrations of 10(-6)-10(-4) M together with ACh modulated the amplitude; accelerated desensitization of currents induced by ACh and increased the final extent of desensitization in a concentration-dependent manner. This finding is in contrast to the suppression and slowing down of desensitization by Phy and 1-methyl-galanthamine observed in Torpedo receptors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fisostigmina
/
Acetilcolina
/
Receptores Nicotínicos
/
Transmisión Sináptica
/
Músculo Esquelético
/
Unión Neuromuscular
Límite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Año:
2006
Tipo del documento:
Article
País de afiliación:
República Checa