[Screening of yeasts producing stable L-lactate cytochrome c oxidoreductase and regulation of enzyme synthesis].
Mikrobiologiia
; 75(1): 29-34, 2006.
Article
en Ru
| MEDLINE
| ID: mdl-16579440
ABSTRACT
Screening of strains producing a stable form of L-lactate cytochrome c oxidoreductase (flavocytochrome b2, FC b2) was carried out among 14 yeast species. Enzyme activity was detected in polyacrylamide gel after the electrophoresis of cell-free extracts. The FC b2 of Hansenula polymorpha, Rhodotorula pilimanae, and Kluyveromyces lactis are characterized by high thermostability; in particular, the FC b2 of H. polymorpha retains its activity and tetrameric structure even after heating at 60 degrees C for 10 min. Constitutive synthesis of FC b2 was observed in H. polymorpha grown on either glucose, ethanol, or glycerol. L-Lactate induces de novo synthesis of FC b2, as proved by the use of cycloheximide, an inhibitor of protein synthesis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Fúngicas
/
Regulación Enzimológica de la Expresión Génica
/
Regulación Fúngica de la Expresión Génica
/
L-Lactato Deshidrogenasa (Citocromo)
/
Saccharomycetales
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
Ru
Revista:
Mikrobiologiia
Año:
2006
Tipo del documento:
Article