Picoliter-volume aqueous droplets in oil: electrochemical detection and yeast cell electroporation.
Electrophoresis
; 27(10): 1977-83, 2006 May.
Article
en En
| MEDLINE
| ID: mdl-16596709
ABSTRACT
An electrochemical detection method was introduced for aqueous droplet analysis in oil phase of microfluidic devices. This method is based on the electrochemical signal difference between aqueous and oil. Applying a low alternating current (AC) voltage to a couple of Au microelectrodes, this method can offer size information and ion concentration range from 0.02 mmol/L to 1 mol/L of tens of picoliter to nanoliter aqueous droplets. Alternatively, applying a relative high AC voltage (18 Vpp) at a frequency of 1 kHz leads to electroporation of yeast cells encapsulated into picoliter droplets. We believe that this simple technique is useful for a number of aqueous droplet-based chemical and biological analyses as well as cell electroporation.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Analíticas Microfluídicas
/
Electroquímica
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Electrophoresis
Año:
2006
Tipo del documento:
Article
País de afiliación:
China