Both msa genes in Renibacterium salmoninarum are needed for full virulence in bacterial kidney disease.
Appl Environ Microbiol
; 72(4): 2672-8, 2006 Apr.
Article
en En
| MEDLINE
| ID: mdl-16597972
ABSTRACT
Renibacterium salmoninarum, a gram-positive diplococcobacillus that causes bacterial kidney disease among salmon and trout, has two chromosomal loci encoding the major soluble antigen (msa) gene. Because the MSA protein is widely suspected to be an important virulence factor, we used insertion-duplication mutagenesis to generate disruptions of either the msa1 or msa2 gene. Surprisingly, expression of MSA protein in broth cultures appeared unaffected. However, the virulence of either mutant in juvenile chinook salmon (Oncorhynchus tshawytscha) by intraperitoneal challenge was severely attenuated, suggesting that disruption of the msa1 or msa2 gene affected in vivo expression.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Salmón
/
Trucha
/
Enfermedades de los Peces
/
Enfermedades Renales
/
Micrococcaceae
/
Antígenos Bacterianos
Límite:
Animals
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos