Benzothiadiazole inhibits mitochondrial NADH:ubiquinone oxidoreductase in tobacco.
J Plant Physiol
; 163(8): 877-82, 2006 Jul.
Article
en En
| MEDLINE
| ID: mdl-16777535
ABSTRACT
An inducer of acquired disease resistance in plants, benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester, exhibited direct, concentration-dependent inhibition of the NADHubiquinone oxidoreductase activity of complex I of the mitochondrial electron transport chain of cultured tobacco cells. The complex I activity was less sensitive to inhibition by salicylic acid, an endogenous activator of acquired disease resistance. Using a dichlorodihydrofluorescein assay, it was found that benzothiadiazole, salicylic acid and the complex I inhibitor rotenone, increased reactive oxygen species production within cells in a concentration-dependent manner. The results indicate that both benzothiadiazole and salicylic acid affect the mitochondria of treated plant cells and result in increased production of reactive oxygen species. The biochemical basis of this response could be related to the inhibition of the NADHubiquinone oxidoreductase activity of complex I that results in channelling of electrons via complex II, with concomitant higher levels of superoxide production.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Tiadiazoles
/
Nicotiana
/
Proteínas Mitocondriales
/
Complejo I de Transporte de Electrón
Idioma:
En
Revista:
J Plant Physiol
Asunto de la revista:
BOTANICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Sudáfrica