Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies.
FEBS Lett
; 580(16): 3799-803, 2006 Jul 10.
Article
en En
| MEDLINE
| ID: mdl-16781713
ABSTRACT
A synthetic peptide corresponding to amino acids (aa) 15-28 of the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein was used to raise polyclonal antibodies in rabbits. This anti-3a N-terminal antibody could detect 3a protein in infected cells, as did an anti-3a C-terminal antibody previously described. The latter targeted the C-terminal cytoplasmic domain of 3a (aa 134-274). The anti-3a N-terminal antibody could detect intracellular 3a as well as 3a expressed on the cell surface. Interestingly, only the anti-3a N-terminal antibody can inhibit SARS-CoV propagation in Vero E6 culture although the binding affinity of the anti-3a N-terminal antibody was lower than the anti-3a C-terminal antibody.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Aminoácidos
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Anticuerpos Antivirales
Límite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2006
Tipo del documento:
Article
País de afiliación:
Suecia