Validation of sensitive human leukocyte antigen-sequence-specific primer and probe typing in forensic DNA examination.
Leg Med (Tokyo)
; 8(4): 203-9, 2006 Jul.
Article
en En
| MEDLINE
| ID: mdl-16807046
ABSTRACT
Validation studies were carried out with the commercially available HLA typing kit using a PCR-SPP (sequence-specific primer and probe) technique. This technique has made it possible to type class I (HLA-A and -B) and class II (HLA-DRB1 and -DQB1) alleles at low-resolution level with total 10 ng of template DNA, in addition to amplify directly from various forms of blood samples without DNA isolation procedure. Experimental examinations with bloodstains smeared on cotton cloth that were a week to 3 months old, bloodstains on gauze stored for 18 years, and buccal cells revealed that this HLA-SPP typing kit is a sensitive and reliable method for forensic investigations.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sondas de ADN de HLA
/
Antígenos HLA-A
/
Cartilla de ADN
/
Genética Forense
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Leg Med (Tokyo)
Asunto de la revista:
JURISPRUDENCIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Japón