Preparation and properties of thermoresponsive bioconjugates of trypsin.
Artif Cells Blood Substit Immobil Biotechnol
; 34(3): 323-36, 2006.
Article
en En
| MEDLINE
| ID: mdl-16809133
ABSTRACT
Covalent attachment of enzymes and other proteins to the smart polymer, poly(N-isopropylacrylamide) [poly (NIPAAm)], has been widely used as a method for the preparation of thermosensitive protein conjugates. In the present study, reversible soluble-insoluble polymer-enzyme conjugates were prepared by conjugating a copolymer of NIPAAm with 5-mol % of 6-acrylaminohexanoic acid to trypsin by the carbodiimide-NHS (N-hydroxysuccinimide) coupling method. Four bioconjugates with different units of enzyme coupled to the matrix were prepared. Increased enzymatic activity in terms of high effectiveness factor (in the range of 3-5) was found in the conjugates. Kinetic parameters for the immobilized and free enzyme were determined. The Vmax/Km value of the enzyme significantly increased on immobilization by the factors in the range of 12-28. The immobilized enzyme also showed stability to autolysis at 50 degrees C.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biopolímeros
/
Tripsina
/
Enzimas Inmovilizadas
Límite:
Animals
Idioma:
En
Revista:
Artif Cells Blood Substit Immobil Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
/
TRANSPLANTE
Año:
2006
Tipo del documento:
Article
País de afiliación:
India