Production of Alexa Fluor 488-labeled reovirus and characterization of target cell binding, competence, and immunogenicity of labeled virions.
J Immunol Methods
; 314(1-2): 30-7, 2006 Jul 31.
Article
en En
| MEDLINE
| ID: mdl-16822520
ABSTRACT
Respiratory enteric orphan virus (reovirus) has been used to study many aspects of the biology and genetics of viruses, viral infection, pathogenesis, and the immune response to virus infection. This report describes the functional activity of virus labeled with Alexa Fluor 488, a stable fluorescent dye. Matrix assisted laser desorption-time of flight analysis indicated that Alexa Fluor 488 labeled the outer capsid proteins of reovirus. Labeled virus bound to murine L929 fibroblasts as determined by flow cytometry and fluorescence microscopy, and the specificity of binding were demonstrated by competitive inhibition with non-labeled virus. Labeled reovirus induced apoptosis and cytopathic effect in infected L929 cells. Mice infected with labeled virus mounted robust serum antibody and CD8(+) T-cell responses, indicating that labeled virus retained immunogenicity in vivo. These results indicate that Alexa Fluor 488-labeled virus provides a powerful new tool to analyze reovirus infection in vitro and in vivo.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Orthoreovirus Mamífero 3
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Coloración y Etiquetado
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Succinimidas
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Virión
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Infecciones por Reoviridae
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos