Quantitative analysis of CCR5 chemokine receptor and cytochrome P450 aromatase transcripts in swim-up spermatozoa isolated from fertile and infertile men.
Arch Androl
; 52(5): 335-41, 2006.
Article
en En
| MEDLINE
| ID: mdl-16873132
ABSTRACT
We determined the CCR5 chemokine receptor and cytochrome P450 aromatase (P450arom) transcript copies number in swim-up sperm isolated from fertile and infertile men. The ejaculates were purified by centrifugation through discontinuous Percoll density gradient and swim-up techniques. RNA was isolated from sperm, treated with DNase I and reverse-transcribed into cDNA. Quantitative analysis of CCR5 and P450arom cDNA were performed by real-time quantitative (RQ-PCR) SYBR Green I analysis. There was a higher content of CCR5 and P450arom transcripts copy number in swim-up sperm of fertile than from infertile donors. The decrease in CCR5 and P450arom transcripts in swim-up sperm may be associated with male infertility.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
ARN Mensajero
/
Aromatasa
/
Receptores CCR5
/
Fertilidad
/
Infertilidad Masculina
Límite:
Humans
/
Male
Idioma:
En
Revista:
Arch Androl
Año:
2006
Tipo del documento:
Article
País de afiliación:
Polonia